In vitro cell migration quantification method for scratch assays
نویسندگان
چکیده
منابع مشابه
In vitro cell migration and invasion assays.
Determining the migratory and invasive capacity of tumor and stromal cells and clarifying the underlying mechanisms is most relevant for novel strategies in cancer diagnosis, prognosis, drug development and treatment. Here we shortly summarize the different modes of cell travelling and review in vitro methods, which can be used to evaluate migration and invasion. We provide a concise summary of...
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Cell invasiveness quantification is of paramount importance in cancer research and is often evaluated in vitro through scratch wound healing assays that determine the rate at which a population of cells fills a gap created in a confluent 2D culture. The quantification of the results of this experiment, however, lacks standardization and is often highly time consuming and user dependent. To over...
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Establishing cell migration assays in multiple different microenvironments is important in the study of tissue repair and regeneration, cancer progression, atherosclerosis, and arthritis. In this work, we developed a miniaturized and massive parallel microfluidic platform for multiple cell migration assays combining the traditional membrane-based cell migration technique and the droplet-based m...
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Scratch assays are often used to investigate potential drug treatments for chronic wounds and cancer. Interpreting these experiments with a mathematical model allows us to estimate the cell diffusivity, D, and the cell proliferation rate, λ. However, the influence of the experimental design on the estimates of D and λ is unclear. Here we apply an approximate Bayesian computation (ABC) parameter...
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ژورنال
عنوان ژورنال: Journal of The Royal Society Interface
سال: 2019
ISSN: 1742-5689,1742-5662
DOI: 10.1098/rsif.2018.0709